Radioimmunoassay for normalized thyroxine with use of Sephadex columns.
نویسندگان
چکیده
We describe a reproducible radioimmunoassay, with use of Sephadex columns, for measuring normalized thyroxine. In comparison with a competitive protein-binding procedure, the present method is more specific because antibody rather than thyroxine-binding globulin is responsible for the competition between endogeneous and tracer thyroxine. In barbital buffer, various concentrations of thyroxine binding pre-albumin and albumin had no influence on the results. Values obtained by the present method correlated well with those by the free thyroxine index and a generally accepted thyroxine normalized method.
منابع مشابه
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متن کاملEvaluation of new kits for the assessment in vitro of thyroid function by determination of serum total thyroxine, free TBG capacity, and free thyroxine index using Sephadex G-25 and '25I-labelled triiodothyronine and thyroxine
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متن کاملtotal thyroxine, free TBG capacity, and free thyroxine index using Sephadex G-25 and '25I-labelled triiodothyronine and thyroxine
SYNOPSIS A trial was carried out on 134 patients of new kits (Ames Co) using columns of Sephadex G-25 for the determination of serum total thyroxine (Tetralute test) and for the indirect estimation of serum free thyroxine-binding globulin capacity (Trilute test). Both new methods were quicker and easier than the reference resin methods and of similar precision. The two measurements when combine...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 23 12 شماره
صفحات -
تاریخ انتشار 1977